Comparison of Hemoglobin Levels between Iron-Supplemented Group and Non-Iron-Supplemented Group in Pregnancy / 대한산부인과학회잡지

OBJECTIVE: Anemia associated with pregnancy is keen of interest to both the mother and the obstetrician and oral iron supplementation for iron deficiency anemia(IDA) during pregnancy is commonpiece. The purpose of this study is to compare the changes in hematologic parameters between iron supplemented and non-iron supplemented women just prior to and 24 hours after delivery. METHODS: A comparative study was conducted concerning the difference in hematologic changes between 30 iron-supplemented and non-iron-supplemented women, who have delivered at Yonsei University Medical School Yongdong Severance Hospital from 1999. 11. 1 from 2000. 1. 18. RESULTS: Between the iron-supplemented and the non-iron-supplemented group, no statistically significant difference was observed for the duration of gestation, neonatal birth weight and maternal age. However, the mean hemoglobin levels were 11.78mg% and 10.34mg% prior to delivery(p<0.001) and 10.52mg% and 9.59mg% 24 hours after delivery, respectively, thus showing a statistically significant decrease in the non-iron-supplemented group(p<0.001). CONCLUSION: Adequate iron supplementation during the antenatal period significantly improves the hematologic status of the mother.

Effects of 12-o-tetradecanoyl-phorbol-13-acetate on beta-hCG secretion by cultured peripheral mononuclear cells during pregnancy / 대한산부인과학회잡지

OBJECTIVE: Peripheral blood mononuclear cells (PBMC) in culture release a biologically active human chorionic gonadotropin (hCG). This effect is detectable during pregnancy with a maximum between the 16th and 19th week. HCG plays an important role for the corpus luteum rescue during the early gestational age and possibly for the immunotolerance. This study was performed to investigate the relationships between the productivity of cultured PBMC of pregnant women and the ability to maintain early pregnancy, and whether 12-o-tetradecanoyl-phorbol-13-acetate (TPA) increases hCG sectetion by cultured PBMCs. MATERIALS AND METHODS: PBMC were obtained from 20 pregnant women between 16th to 19th week of gestation , and cultured with TPA. Culture cells were harvested and hCG mRNA were extracted and RT-PCR were performed. Culture supernatants were collected and hCG concentration were determined by commercial RIA methods. RESULTS: The mean age was 31.0 years old, 19 of 20 (95%) pregnant women's PBMC secereted hCG and expressed hCG mRNA, but in control group exept male hepatitis B patient, none of them produced hCG. TPA activated expression of hCG in PBMC in linear manner. CONCLUSION: Pregnant women's cultured PBMC secreted hCG, but not in non-pregnant or male. We could confirm the mRNA of hCG in PBMC as well in the placental control. The productivity of hCG in PBMC might be closely related with maintenance of early pregnancy.